Hi CellProfiler Team,
I have cropped one of my pictures down to two nuclei and would like the program to identify both of them as primary object; however, the entire right nuclei is not being identified, just its intense chromatin in the middle.
I realize it is probably because the right nuclei is very faint compared to the one on the left…how can I get CellProfiler to identify the whole thing?
I attached the pipeline also, in case you need it.
DefaultOUT__7PIPE.mat.cp (8.02 KB)