I also need your help - though I’ve really tried to manage creating a new pipeline for my image analysis I stuck in too many points to get it finished.
As Overview: I want to measure the length of these cilia - stained in red.
This image is originally an .lsm, so I want to make a Z projection, crop it and just analyse the length of the cilia in the middle of the lumen (green)- and finally get the data within a spreadsheet.
I’m really clueless! So pleeease help me!