2016.09.26 - 488-beta-caenin.tif.tif (768.2 KB)
I have been struggling to find a way to segment the cells in this image. The cells have been stained with anti-beta catenin antibody. My goal is to segment the cells and then analyze them for morphological data, such as shape, AR, circularity, etc.
Doing this by hand takes forever, so I wanted to find a quicker, at least semi automated way to do it. One thing I have used so far is StarDist, but I am not satisifed with the quality of the segmentation. I have also tried segmenting through CellProfiler and ilastik, but I can’t figure out how to get these software to recognize the cells, instead of just highlighting the boundaries.
Also, any advice on what filters to use would be great too. I am very new to image analysis (I sort of got thrown into it due to COVID) and so I finding the best way to do anything is very confusing and difficult for me.
Any help would be much appreciated! Thank you!