Hi all the experts in the ImageJ community,
I took 3D-stack images examining distribution of my protein and saw clustering of proteins in the experimental group compared to control group (Please see the screenshot image below).
(Left: control; Right: Expt group)
I would like to 1) Segment the puncta-shaped objects in 3D, and 2) measure the size, number, mean intensity, … of detected objects.
First I tried with 3D object Counter, but it does not segment the objects as I wanted them to be…
Could you please guide me what approaches I can take to achieve my goal?
I am uploading 3D Tiff images from my experiments.
Thank you very much for your guidance in advance,