I’ve been trying for a while to calculate and apply the correct illumination in order to use the modul apply threshold. However, doesn’t matter which set up I use in IlluminationCalculate, they all seem to yield the same result. And its considering to much of the background as signal. Do you have any suggestions as to how I can change the settings (I have already consulted the examples and tutorials for help)?
Here is the pipeline I’m using: Bgd.cppipe (24.0 KB)
and an example pic: https://drive.google.com/open?id=19VmWf7OGuxiMv6Sqh8tO_LD4oG4LxcDH