Some time ago i developed a pipline to safely measure the CSA of muscle’s fibers.
The program work pretty fine, confirming that Cell Profiler is a powerful program, but i have problems with some .nd2 files.
I have two .nd2 files containing tritc and dapi channels; one is correctly running through the program, while the other is not correctly processed.
The two images are from the same sample and the only difference is that the first one is over-saturated, while the second one is far better.
Probably i’m overlooking something, but nonetheless i’m buffled by this problem.
This is the pipeline CSA_3.0.cpproj (706.3 KB)
This is the working image: CTRL
This is the not working one: PRDN
Thank you for the help!