Hi, I have been having a look through the forums and cant seem to find what I am looking for. I have a large number of images of muscle fibers from fish. Unlike mammals the fibers are constantly being recruited and enlarged. I am looking to measure all of the fibers in each image so that I can compare between fish that have been exposed to different treatments.
I have included an example of what the images look like. Sometimes there is some white between the cells from shrinkage like in the top left of the image but this is not always the case. I have the original images as true tif files so separating out each channel is easy.
Last time I did this exercise I manually traced round each cell and then scanned and analyzed particles which worked very well but I am keen to avoid the weeks of tracing if possible.
Many thanks for any suggestions.