I would like to use @StephanPreibisch’s Multiview Reconstruction plugin to register different time-points of a 3D SPIM movie. The individual time-points are single files with single views (they are fused already from multiple views) and contain nuclei that I would like to use for identifying the transformation between time-points.
I was watching the tutorial here: http://imagej.net/Multiview-Reconstruction, but the time-series case is not covered. So I tried on my own using Match against one reference timepoint, but I got an error that I do not understand (see below). I guess it has to do the settings Fix Tiles and Map back tiles. I also attached the log and xml file.
log_dataset.zip (2.3 KB)
Is there somewhere a tutorial for “nuclei-based time-series registration”, explaining the Fix Tiles and Map back tiles options?
Using spimdata version: 0.9-revision
Using spimreconstruction version: 4.0.0
angles selected: 0
channels selected: 0
illuminations selected: 0
Timepoints selected: 9, 10
registering channel: 0 label: ‘beads’
Registration algorithm: Fast 3d geometric hashing (rotation invariant)
Registration type: TO_REFERENCE_TIMEPOINT
Channels to process: 1
Following tiles are fixed:
Angle:0 Channel:0 Illum:0 TimePoint:9
Following tiles are reference tiles (for mapping back if there are no fixed tiles):
(Tue Jun 28 10:17:35 CEST 2016): Starting registration
(Tue Jun 28 10:17:35 CEST 2016): Finding correspondences for subset: reference timepoint ( 9, id=9) registration: 10(id=10)
(Tue Jun 28 10:17:36 CEST 2016): [TP=10 angle=0, ch=0, illum=0 >>> TP=9 angle=0, ch=0, illum=0]: Not enough correspondences found 3, should be at least 12
(Tue Jun 28 10:17:36 CEST 2016): Number of Candidates: 3
(Tue Jun 28 10:17:36 CEST 2016): Number of Inliers: 0
(Tue Jun 28 10:17:36 CEST 2016): Fixing view-tile (viewSetupId = 0)