I’m having a problem using the MultipleKymograph plugin with the tsp050706.txt macro and was wondering if anyone could help! I have successfully used MultipleKymograph and ‘read velocities from tsp’ (a feature of the tsp050706.txt macro) to measure the velocity on some time series images. However, when I use MultipleOverlay in conjunction with MultipleKymographs I can no longer use the ‘read velocities from tsp’ macro. When I try and measure the velocity of a line ROI, I keep getting an error that reads ‘Please use macro kymograph first’.
Is anyone familiar with this error?