Multi-colour kymograph

Hello everybody,

I´m trying to make a coloured kymograph from a movie of endosomes with two different labels. Both markers show co-transport in the movie and I would like to have a kymograph where the lines are shown in the same colours as in the movie. So I have one protein tagged with gfp and the other in red and I want to have green lines if the gfp-tagged protein is moving alone, red lines if the other one is moving alone and yellow lines if they move together. I´m familiar with making kymographs from a movie with one colour in black-and-white and I was also able to split the movie to get a kymograph for each signal but can anybody tell me how to make one merged kymograph with both colours?

Thanks for your help!
Best regards

Hi,

You can merge black and white pictures back by using Colors->Merge channels.

Or use Kymoreslicewide plugin, it works with stacks and multichannel stacks

Cheers,
Eugene

Wow thank you so much! Problem solved :slight_smile: