Motorized Stage quits during time course

Hi all,

This is a cross-post from microlist

We have acquired a used Olympus IX81 with a Sigma Koki XY motorized stage. We are doing time courses with multi ROI, and after ~4 time points the stage becomes unresponsive. We are running with micro-manager 2.0. Has anyone encountered a stage suddenly becoming unresponsive to a computer but still ok with the joystick?

# Generated by Configurator on Mon Nov 30 14:06:55 EST 2020

# Reset
Property,Core,Initialize,0

# Devices
Device,COM1,SerialManager,COM1
Device,COM6,SerialManager,COM6
Device,OlympusHub,Olympus,OlympusHub
Device,Objective,Olympus,Objective
Device,LightPath,Olympus,LightPath
Device,Shutter1,Olympus,Shutter1
Device,Shutter2,Olympus,Shutter2
Device,TransmittedLamp,Olympus,TransmittedLamp
Device,FluorescenceLamp,Olympus,FluorescenceLamp
Device,ManualFocus,Olympus,ManualFocus
Device,AutoFocusZDC,Olympus,AutoFocusZDC
Device,AutoFocus Stage,Utilities,AutoFocus Stage
Device,TUCam,Tucsen_x64,TUCam
Device,XYStage,SigmaKoki,XYStage

# Pre-init settings for devices
Property,OlympusHub,Port,COM1
Property,XYStage,Move per step(micron),2.0000
Property,XYStage,Port,COM6

# Pre-init settings for COM ports
Property,COM1,AnswerTimeout,500.0000
Property,COM1,BaudRate,19200
Property,COM1,DataBits,8
Property,COM1,DelayBetweenCharsMs,0.0000
Property,COM1,Fast USB to Serial,Disable
Property,COM1,Handshaking,Off
Property,COM1,Parity,Even
Property,COM1,StopBits,2
Property,COM1,Verbose,1
Property,COM6,AnswerTimeout,500.0000
Property,COM6,BaudRate,9600
Property,COM6,DTR,Disable
Property,COM6,DataBits,8
Property,COM6,DelayBetweenCharsMs,0.0000
Property,COM6,Fast USB to Serial,Disable
Property,COM6,Handshaking,Off
Property,COM6,Parity,None
Property,COM6,StopBits,1
Property,COM6,Verbose,1

# Hub (parent) references

# Initialize
Property,Core,Initialize,1

# Delays

# Focus directions
FocusDirection,ManualFocus,0
FocusDirection,AutoFocus Stage,0

# Roles
Property,Core,Camera,TUCam
Property,Core,Focus,ManualFocus
Property,Core,AutoShutter,1

# Camera-synchronized devices

# Labels
# Objective
Label,Objective,5,Position-6
Label,Objective,4,Position-5
Label,Objective,3,Position-4
Label,Objective,2,Position-3
Label,Objective,1,Position-2
Label,Objective,0,Position-1
# LightPath
Label,LightPath,1,Side Port
Label,LightPath,0,Eye
# TransmittedLamp
Label,TransmittedLamp,1,On
Label,TransmittedLamp,0,Off
# FluorescenceLamp
Label,FluorescenceLamp,1,On
Label,FluorescenceLamp,0,Off

# Configuration presets
# Group: Objective type ZDC
# Preset: UPlanSApo60XO
ConfigGroup,Objective type ZDC,UPlanSApo60XO,AutoFocusZDC,ObjectiveTypeSetting,UPlanSApo60XO

# Preset: LCPlanFl 20X
ConfigGroup,Objective type ZDC,LCPlanFl 20X,AutoFocusZDC,ObjectiveTypeSetting,LCPlanFl 20X

# Preset: LUCPlanFlN20X
ConfigGroup,Objective type ZDC,LUCPlanFlN20X,AutoFocusZDC,ObjectiveTypeSetting,LUCPlanFlN20X

# Preset: UPlanFlN60XOI
ConfigGroup,Objective type ZDC,UPlanFlN60XOI,AutoFocusZDC,ObjectiveTypeSetting,UPlanFlN60XOI

# Preset: UPlanFlN100XO
ConfigGroup,Objective type ZDC,UPlanFlN100XO,AutoFocusZDC,ObjectiveTypeSetting,UPlanFlN100XO

# Preset: UPlanFlN100XOI
ConfigGroup,Objective type ZDC,UPlanFlN100XOI,AutoFocusZDC,ObjectiveTypeSetting,UPlanFlN100XOI

# Preset: PlanApo 60X O-LSM
ConfigGroup,Objective type ZDC,PlanApo 60X O-LSM,AutoFocusZDC,ObjectiveTypeSetting,PlanApo 60X O/LSM

# Preset: PlanApo 60XO3
ConfigGroup,Objective type ZDC,PlanApo 60XO3,AutoFocusZDC,ObjectiveTypeSetting,PlanApo 60XO3

# Preset: UPlanFl 100XO3
ConfigGroup,Objective type ZDC,UPlanFl 100XO3,AutoFocusZDC,ObjectiveTypeSetting,UPlanFl 100XO3

# Preset: UPLFLN60X
ConfigGroup,Objective type ZDC,UPLFLN60X,AutoFocusZDC,ObjectiveTypeSetting,UPLFLN60X

# Preset: UPlanSApo40X
ConfigGroup,Objective type ZDC,UPlanSApo40X,AutoFocusZDC,ObjectiveTypeSetting,UPlanSApo40X

# Preset: PlanApo 60X O TIRFM-SP
ConfigGroup,Objective type ZDC,PlanApo 60X O TIRFM-SP,AutoFocusZDC,ObjectiveTypeSetting,PlanApo 60X O TIRFM-SP

# Preset: None
ConfigGroup,Objective type ZDC,None,AutoFocusZDC,ObjectiveTypeSetting,None

# Preset: UplanFlN40X
ConfigGroup,Objective type ZDC,UplanFlN40X,AutoFocusZDC,ObjectiveTypeSetting,UplanFlN40X

# Preset: UPlanFlN60X
ConfigGroup,Objective type ZDC,UPlanFlN60X,AutoFocusZDC,ObjectiveTypeSetting,UPlanFlN60X

# Preset: UApo 40XOI3 -340
ConfigGroup,Objective type ZDC,UApo 40XOI3 -340,AutoFocusZDC,ObjectiveTypeSetting,UApo 40XOI3 /340

# Preset: UPlanSApo20X
ConfigGroup,Objective type ZDC,UPlanSApo20X,AutoFocusZDC,ObjectiveTypeSetting,UPlanSApo20X

# Preset: UPlanFl 40X O-SP
ConfigGroup,Objective type ZDC,UPlanFl 40X O-SP,AutoFocusZDC,ObjectiveTypeSetting,UPlanFl 40X O-SP

# Preset: UApo40XW
ConfigGroup,Objective type ZDC,UApo40XW,AutoFocusZDC,ObjectiveTypeSetting,UApo40XW

# Preset: PlanApo40XW-LSM
ConfigGroup,Objective type ZDC,PlanApo40XW-LSM,AutoFocusZDC,ObjectiveTypeSetting,PlanApo40XW/LSM

# Preset: UPLSAPO60XO
ConfigGroup,Objective type ZDC,UPLSAPO60XO,AutoFocusZDC,ObjectiveTypeSetting,UPLSAPO60XO

# Preset: UPlanApo 40X OI3
ConfigGroup,Objective type ZDC,UPlanApo 40X OI3,AutoFocusZDC,ObjectiveTypeSetting,UPlanApo 40X OI3

# Preset: LUCPlanFlN60X
ConfigGroup,Objective type ZDC,LUCPlanFlN60X,AutoFocusZDC,ObjectiveTypeSetting,LUCPlanFlN60X

# Preset: LCPlanFl 60X
ConfigGroup,Objective type ZDC,LCPlanFl 60X,AutoFocusZDC,ObjectiveTypeSetting,LCPlanFl 60X

# Preset: LUCPLFLN40X
ConfigGroup,Objective type ZDC,LUCPLFLN40X,AutoFocusZDC,ObjectiveTypeSetting,LUCPLFLN40X

# Preset: UAPO40XO Iris3-340
ConfigGroup,Objective type ZDC,UAPO40XO Iris3-340,AutoFocusZDC,ObjectiveTypeSetting,UAPO40XO Iris3/340

# Preset: UPLFLN100XOI
ConfigGroup,Objective type ZDC,UPLFLN100XOI,AutoFocusZDC,ObjectiveTypeSetting,UPLFLN100XOI

# Preset: PlanApoN60XO
ConfigGroup,Objective type ZDC,PlanApoN60XO,AutoFocusZDC,ObjectiveTypeSetting,PlanApoN60XO

# Preset: UApoN100XOTIRF
ConfigGroup,Objective type ZDC,UApoN100XOTIRF,AutoFocusZDC,ObjectiveTypeSetting,UApoN100XOTIRF

# Preset: PlanApo 100XO3
ConfigGroup,Objective type ZDC,PlanApo 100XO3,AutoFocusZDC,ObjectiveTypeSetting,PlanApo 100XO3

# Preset: UPlanApo60XW
ConfigGroup,Objective type ZDC,UPlanApo60XW,AutoFocusZDC,ObjectiveTypeSetting,UPlanApo60XW

# Preset: UPlanAPO 20X
ConfigGroup,Objective type ZDC,UPlanAPO 20X,AutoFocusZDC,ObjectiveTypeSetting,UPlanAPO 20X

# Preset: UPlanSApo100XO
ConfigGroup,Objective type ZDC,UPlanSApo100XO,AutoFocusZDC,ObjectiveTypeSetting,UPlanSApo100XO

# Preset: UPlanFl40X
ConfigGroup,Objective type ZDC,UPlanFl40X,AutoFocusZDC,ObjectiveTypeSetting,UPlanFl40X

# Preset: LUCPlanFlN40X
ConfigGroup,Objective type ZDC,LUCPlanFlN40X,AutoFocusZDC,ObjectiveTypeSetting,LUCPlanFlN40X

# Preset: LCPlanFl 40X
ConfigGroup,Objective type ZDC,LCPlanFl 40X,AutoFocusZDC,ObjectiveTypeSetting,LCPlanFl 40X

# Preset: UPlanAPo 100XOI3
ConfigGroup,Objective type ZDC,UPlanAPo 100XOI3,AutoFocusZDC,ObjectiveTypeSetting,UPlanAPo 100XOI3

# Preset: LUCPLFLN 20X
ConfigGroup,Objective type ZDC,LUCPLFLN 20X,AutoFocusZDC,ObjectiveTypeSetting,LUCPLFLN 20X

# Preset: UApo 40X3 -340
ConfigGroup,Objective type ZDC,UApo 40X3 -340,AutoFocusZDC,ObjectiveTypeSetting,UApo 40X3 /340

# Preset: UPlanFlN20X
ConfigGroup,Objective type ZDC,UPlanFlN20X,AutoFocusZDC,ObjectiveTypeSetting,UPlanFlN20X

# Preset: UPLFLN100XO2
ConfigGroup,Objective type ZDC,UPLFLN100XO2,AutoFocusZDC,ObjectiveTypeSetting,UPLFLN100XO2

# Preset: UPlanApo60XW-IR
ConfigGroup,Objective type ZDC,UPlanApo60XW-IR,AutoFocusZDC,ObjectiveTypeSetting,UPlanApo60XW/IR

# Preset: ApoN60XOTIRF
ConfigGroup,Objective type ZDC,ApoN60XOTIRF,AutoFocusZDC,ObjectiveTypeSetting,ApoN60XOTIRF

# Preset: LUCPlanFl 40X
ConfigGroup,Objective type ZDC,LUCPlanFl 40X,AutoFocusZDC,ObjectiveTypeSetting,LUCPlanFl 40X

# Preset: UPlanFlN40XO
ConfigGroup,Objective type ZDC,UPlanFlN40XO,AutoFocusZDC,ObjectiveTypeSetting,UPlanFlN40XO

# Preset: UPlanFl 20X
ConfigGroup,Objective type ZDC,UPlanFl 20X,AutoFocusZDC,ObjectiveTypeSetting,UPlanFl 20X

# Preset: UPlanSApo60XW
ConfigGroup,Objective type ZDC,UPlanSApo60XW,AutoFocusZDC,ObjectiveTypeSetting,UPlanSApo60XW

# Preset: UApo 20X3 -340
ConfigGroup,Objective type ZDC,UApo 20X3 -340,AutoFocusZDC,ObjectiveTypeSetting,UApo 20X3 /340

# Preset: UPLanFl 60XOI3
ConfigGroup,Objective type ZDC,UPLanFl 60XOI3,AutoFocusZDC,ObjectiveTypeSetting,UPLanFl 60XOI3

# Preset: UPLSAPO40X2
ConfigGroup,Objective type ZDC,UPLSAPO40X2,AutoFocusZDC,ObjectiveTypeSetting,UPLSAPO40X2

# Preset: SLCPlanFl 40X
ConfigGroup,Objective type ZDC,SLCPlanFl 40X,AutoFocusZDC,ObjectiveTypeSetting,SLCPlanFl 40X

# Preset: UPlanApo 60X
ConfigGroup,Objective type ZDC,UPlanApo 60X,AutoFocusZDC,ObjectiveTypeSetting,UPlanApo 60X


# Group: light
# Preset: 1
ConfigGroup,light,1,TransmittedLamp,ClosedPosition,1

# Preset: 0
ConfigGroup,light,0,TransmittedLamp,ClosedPosition,0


# Group: Measure off set ZDC
# Preset: Off
ConfigGroup,Measure off set ZDC,Off,AutoFocusZDC,MeasureOffset,Off

# Preset: Now
ConfigGroup,Measure off set ZDC,Now,AutoFocusZDC,MeasureOffset,Now


# Group: Channel

# Group: ZDC search range 
# Preset: NewPreset
ConfigGroup,ZDC search range ,NewPreset,AutoFocusZDC,SearchRange,0.0000


# Group: System
# Preset: Startup


# Group: control
# Preset: Computer
ConfigGroup,control,Computer,OlympusHub,Control,Computer

# Preset: Manual + Computer
ConfigGroup,control,Manual + Computer,OlympusHub,Control,Manual + Computer



# PixelSize settings

Ah! Saw your microforum post first. Let’s continue here if needed.