I have a big set of images (bright-field), and I need to get data on cell morphology for each (size, roundness, aspect ratio, etc.). I am new to cellprofiler, and I have tried the “Load an example pipeline” option…the result from this analysis is exactly what I am looking for: an excel sheet with all the morphological information about the cell.
My questions are:
- Is it possible to use cellprofiler for unstained (bright-field) images?
- How do I run the same pipeline as the one in the “example pipeline” but on my own set of images?
Would appreciate an answer in simple terms as I am a beginner in image analysis.