I have four separate channel of individual biological importance. One of them is DAPI. I want to segment Nuclei as Primary object and quantify intensity in remaining three channels. I don’t know how to call/label four images for further processing. Are we only limited to process 3 channels using RGB conversion?
Following are my steps. I need help in step 2
Step 1: Converted to Well, Position, and plate metadata using following RE.
Step 2: Now I want to call Channel 1 as my nuclei and perform primary object (I don’t know how to choose “ChannelName” within “IdentifyPrimaryObjects” ruleset)?