Modifying Trackmate to Export Mean Intensity of Non-Zero Pixels?

Me again! I’m wondering if anyone has sat down to develop a new spot statistic for mean intensity in each channel, in which the mean is only calculated from non-zero pixel values only. I’m testing segmenting my images to eliminate background, and deleted everything outside of my cell, but now the mean intensity values are dragged down by the black. I’m trying to learn more about coding (definitely a novice) and looked through the Wiki for Extending Trackmate but I have no idea how to start. Or does anyone know of a Trackmate or FIJI workaround?

Hello @sparkerino

I sincerely think that for this there is no workaround and you need to implement your own spot feature calculation…