I’m working with cellprofiler to identify the proteintranslocation between cytoplasm and nucleus. Right now I have 3 different models and I want to know if there could be some other, maybe better models. For all experiments I used epithelial-cells with nuclei and antibody staining with 60x magnification and I want to measure & process the IntegratedIntensity.
My first simple model is the standard propagation model which is explained at the CellProfiler page in detail.
In a second, more complex, model I included the IlluminationCorrection feature to improve the cellshapes.
The last one only includes the Distance-N module for second identification twice done, because I found that these “donut-method” is often done in the industry to get quick results.
So maybe one of you know another method that fit in here.
Anywhere, is there a common method to evaluate all the models to find the best one ? (beside RSS, AIC)
P.S.: Thanks for this programm =)