This question is not about micro-manager specifically, but maybe micro-manager users and DIY microscopists could answer. Please move/delete this post if it’s off topic.
I often work with FLIR (Point Grey) cameras. They’re very good, even for fluorescence.
Now, I am building a fluorescence module inside a 37ºC incubation chamber, to run timelapse imaging of weakly fluorescent bacterial colonies on Petri dishes. In these conditions, FLIR cameras become limited. Their dark current is low so long exposure (typically 1 second) and heat are not a problem for the background noise, for most of the pixels. However, at 37ºC and 1 second exposure, there are many “hot pixels” that give the image a salt and pepper look.
I’m looking for an alternative for a cheap-ish (I mean not an ORCA-Flash4.0) camera with cooling. Ideally, I need the same specs as a FLIR camera (2/3" or 1" sensor, 12 or 16 bits, 60-70 dB dynamic range, acquisition from 1 ms to 10 sec; I don’t need high FPS), with a cooling of 30-40ºC. Also, the camera needs to be controlled from micro-manager.
I looked into ZWO and ATIK, but it is not clear if there are MM device adapters for these cameras (I’m waiting for answers from them). Is there a manufacturer that I overlooked? Does anyone have recommendations?
Thanks for your help,