I’m a new user of Cell profiler, and therefore have no expertise in building a suitable pipeline for my needs. I was wondering if anyone might have a pipeline already made for the analysis I need to do, or whether anyone could advise me on which modules to use.
The overexpression of our protein of interest leads to a shift in the distribution of the mitochondria from a diffuse cytoplasmic distribution to a perinuclear one. We would like to enumerate this change in distribution, so that we can then try and reverse the phenotype experimentally using siRNAs, compounds, or to compare mutants of our protein of interest to the wild-type protein.
I attach two images to represent the sort of phenotype I’d like to analyse.
Many thanks in advance!