Hi! I need help, if possible.
Firstly I will analyze fish intestinal samples by transmission electron microscopy with objective to measure the density of the microvilli. In several articles it is mentioned the use of ImageJ to make this analysis, but they are not clear.
I saw this in one of them: Briefly, micrographs were converted to 8-bit, and the ratio of white / black (i.e., foreground / background) was calculated to give a microvilli density measure (arbitrary units [a.u.]).
Can anybody help me? Thanks