I am posting this topic, as I was not sure if the method and modules I was using was correct, despite going through the manual and other posts.
What I need to measure: Microvessel density in the image below. The vessels are stained brown.
Method I am using: Manually outline the vessels (using identify objects manually), measure area of the outlined vessels (using measure image area occupied). I have an output as indicated below:
Objects or Image Area Occupied Perimeter Total Area
Vessels 18573 4252 4915200
The total area is the area of the image, right?
I understood from one of your discussions that the measurements are in (pixel)2. I require the area in mm2 or µm2. Therefore, I multiplied the area in pixels by 102.01 (1 pixel 2= 102.1 um2).
- I would like to know if the method described above is correct.
- Outlining each vessel is difficult when there are several vessels. Is there a module to select all vessels together (based on the staining intensity) ?
Please help me with these.