I am writing a script to automate the identification of a slice most in-focus within a stack of fluorescence microscopy images. Tried several plugins available and got best results with the Microscope Focus Quality plugin.
It generates a PROBABILITIES STACK from which the first slice seems to match well the evaluation of the focus quality that can be overlay on the image of interest. But I don’t seem to find anywhere the meaning of the other slices in the PROBABILITIES STACK. Does anyone know?