We are using MicroManager 2.0 to control a Nikon Ti for multi-position time-lapse experiments. Fairly frequently we find that MicroManager quits at some point during the experiment. This has mostly been at night so we were unable to diagnose the cause but yesterday it happened during the day and we saw the following error message:
Error in device “TiPFSStatus” PFS focusing timed out (timeout set to 5000 ms) (25001)
Yet the cells were still in focus.
Can anyone advise?