I’m trying to record from two Photometrics Evolve 128 cameras using MicroManager, and after promising start, I’ve encountered a peculiar problem. I’ll start with the most recent 1.4 version.
When I run live view, everything is great, frame rate is as expected, etc. However, when I run multi-dimensional acquisition (mda), with everything but the box determining number of samples unchecked (i.e., recording to RAM and no extra stuff that could slow it down), the live view that opens is pretty slow. What I found happens is that it does record the frames it should, but then keeps displaying them over a fairly long period in slow-motion before they can be stored. For example, recording 10k frames at a certain condition records 22 seconds, but the live view showing what’s recorded is hanging there for over a minute, slowly presenting the images at around 100 FPS, while the actual recording rate is around 400 FPS (this is preventing me from taking another recording soon after the first one, which is unfortunately a critical limitation for our experiments). This happens consistently across camera parameters, binning, etc. It sounds to me quite like the issue described here: http://micro-manager.3463995.n2.nabble.com/quot-Slow-display-quot-td7579145.html"m but I could not find a place to toggle the “slow display” mentioned there.
I also tried version 2.0 of MicroManager, but there I had even less success - Live view is fine, but once I try to do acquisition, the message window first says “received 0 out of 1 images”, which gradually changes to “5 out of 1 images”, but the window where the images recorded should be played remains empty, hanging there with “Waiting for Image”, not progressing further.
I’d be extremely grateful for any assistance with this.