Method for colocalisation

iytuty_Series016.tif (780.6 KB)

Hello,

I am learning how to use Image J and I need help…
I need to count the number of colocalisation between a extracellular matrix and a protein (
colocalization = synaptic connection)

I have tried some tutorials and plugins like Coloc 2 but I am not sure about the results and the method…
If someone can explain me a detailled method for this kind of analyze…

Thank you !

Here are some links I collected for a general course I gave in the past which might be helpful:

Forum archive:

http://forum.imagej.net/search?q=colocalization

ImageJ Plugins:

http://imagejdocu.tudor.lu/doku.php?id=plugin:analysis:jacop_2.0:just_another_colocalization_plugin:start

Links:

Literature:

http://www.physiology.org/doi/abs/10.1152/ajpcell.00462.2010

Tutorials (and datasets):

http://www.med.unc.edu/microscopy/resources/learning/colocalization-tutorial/Colocalization%20Overview

Presentations:

https://www.biodip.de/w/images/f/fa/QuantitativeColocAnalysis-10-2011.pdf

Help:

https://www.researchgate.net/post/How_to_quantify_colocalization_of_dual-color_immunofluorescence_image

Principles:

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