I am an MD new to the lab, so I apologize if I am missing something basic. I have been having trouble tracing my cells well. I have tried setting a threshold but haven’t had much success, and when that works it is often for a specific image only and not replicable for other images that may be brighter or darker. Is there a better way? I am using Hoechst stain for my nuclei and cell trackers for cells.
pipeline for N and C CMFDA.cp (5.6 KB)