Hi guys, could you help me add a rule to my pipeline to merge two objects in close proximity into one? I realize that this should be possible under IdentifyPrimaryObjects, but whatever I try I still keep 2 distinct objects (see objects 11-12 and 17-19 in the 2nd attached image). I have tried several things: I set ‘method to distinguish between’ and ‘draw a line between objects’ to ‘intensity’ and then I varied both the ‘size of smoothing filter’ between 1-2000 and ‘suppress local maxima that are closer than this minimum allowed distance’ also between 1-2000 (since I read somewhere that this affects whether or not objects are merged) but to no avail. Am I doing something wrong here?
Some more info on my pipeline: I select objects, then shrink them to a dot, then expand them to the size of the outlines in the attached image (to later measure in a different channel).
Something else I tried is to unify objects based on proximity, but then even though they become one object, the outline follows both their centers as you can see in the first attached image.
Great program btw!
PS. is there an option to change from ‘dot’ to ‘comma’ as decimal separator when exporting to .csv? I have to replace them manually now to avoid excel interpreting the values incorrectly.