I’m trying to measure the distribution of a bunch of puncta and splotches of the Golgi within the cytoplasm, i.e., compact or spread throughout the cytoplasm. I am able to identify the Cell and to find the Golgi splotches, and to relate the two. My plan was to merge all the Golgi objects within each cell into one object per cell, and measure the area of the bounding ellipsoid.
I seem to be stuck at how to merge the Golgi objects into a single object. I’ve tried a few approaches, but none seem to work very well.
This is what I’ve tried that almost works:
Identify a Tertiary Object using Cell as the larger object and Golgi as the smaller object. This creates a single object that is NotGolgi. Then I repeat the process, using Identify Tertiary Object with Cell as the larger object and NotGolgi as the smaller object.
This almost works, except it includes the perimeter of the cell in the final object, instead of just the internal spots. I’ve tried to fix this by next Shrinking the Cell by 2 pixels and using it to Mask the final object. This almost works, but still leaves a few spots at the perimeter of the cell, and that messes up the measurements.
Is there a better way to turn all of the Golgi objects for each into a single object and measure the area of the bounding ellipsoid (or some similar measure of the spread of the Golgi)?