I am using cell profiler to quantify TUNEL stains on high magnification images.
Just using grayscale/thresholding and size criteria I’m able to identify the stain +ve nucleii quite well.
The only problem is for some nucleii it is identifying them as 2-3 separate objects that are touching or very,very close.
Attached is the image w. objects.
The TUNEL+ve count would be more accurate if I could merge the touching/very close objects.
one of the previous posts mention ‘unify’ module in previous versions?
-is that available or are there any other ways to do this?
thanks for making this software available - I think it is going to save us a lot of mindless labour with more reproducible results.