I’m new to ImageJ and don’t really know how to solve my problem.
I want to measure the cell sizes/areas and to count the cells, but because of the low contrast between the cells and the base area and the light reflection of the cells the threshhold can’t distinguish between cell areas and the base area. The function “analyze particles” then just creates me some big and wrong ellipses because the threshholded picture isn’t clear enough.
I’ve marked exemplary some of the cells as ellipses.
I would be very grateful if you could help.