I am working on some TEM image data-sets containing lattice pattern of 2D protein crystals. I am interested to find out the area of the regions that contain lattice pattern of crystals. In order to measure area I’m using this approach in ImageJ :
Selection of crystal lattice pattern by freehand / polygon selection method >> Analyze >> Measure
At output I am getting result of areas, such as let say ‘‘Area=842842’’ along with values of Mean, Min, Max etc.
However, I am unable to understand few things and have following queries:
- Does this value of area depicts the number of pixels? (means in that particular section there are 842842 pixels?) or something else?
- Secondly, If it depicts simply the area, then what is the unit describing this value? µm, cm, m, m2?
- Lastly, as I had captured the TEM images at 100 nm resolution. So how may I convert above area value in terms of nm resolution?
My questions may be too basic as I am new in ImageJ forum, sorry for that. But, I would highly appreciate if someone help to make me to understand above questions.
P.S. Image is attached here for reference.