I want to analyze the image below by first binarizing it and then using the Skeletonize and Analyzeskeleton plugins developed by @iarganda
8:5:19 onh.bmp (1.0 MB)
I then want to do the same procedure on subsequent images after stimulus X and compare them to the original image in order to see if any change has occurred. I was planning on comparing number of branches and sum of the length of all skeletons.
My question is whether this method is valid or if this images like these are too complex to get an accurate and consistent reading for this method.
A follow up question I have is: Do y’all have a method for measuring angiogenesis that you recommend?