Measuring polarity within a cell

I am looking at the morphology of a lipid membrane within differentiating cells, and would like to be able to categorise a heterogeneous population based on the polarity of the membrane within the cell (The hypothesis being that as the cell differentiates the lipid mass polarises to one side of the cell). I would like to do this in 3D, but cannot find any plugins to help me analyse my images.

Would anyone be able to suggest a plugin that I can use?

Hi @TJO735,

Sample images are always welcome!