I wanted to use your program to quantify nuclear intensities of a transfected protein in immunofluorescence samples. For this, I stained my protein of interest with Antibody/Alexa488 and added DAPI as a counterstaining. I tried to adapt your example pipeline “ExamplePercentPositive” for my purposes.
What I need, are signal intensities of my protein in the nucleus of every single cell that is transfected. That means, I have more than one picture to get enough data for statistical analysis. Is it possible to make such a pipeline, that shows me all single intensities?
Thanks a lot!