I am having trouble with optimizing my pipeline. I am trying to measure the image intensity in only one channel (red) of a merged .tif picture (blue and red) but I’d like to count the number of objects (blue) as well. Is this possible? I really just need the fluorescence intensity in the red channel of a merged image (without having to unmerge each one) but I don’t see how to change the settings of the module to do this?
Thank you very much for your time and help,