I’m new to ImageJ and I have no idea what I’m doing!
So far I’ve been trying to work out the fluorescent intensity of Fura-2 at different time points on Image J.
What I’ve been doing: For each Image I’ve been measuring the ROI and averaging the values for each image and then I’ve been subtracting the background, which has been different for each image.
Is it normal to have different background for each image? Am I doing the right thing?
28-08-2019_1_Fl before inc.tif (284.0 KB)