Sample image and/or code
I was happy to discover this forum and after a short browsing I’m sure you will have a solution for my issue. While my task is relatively simple, the images/results I have to work with are a bit more challenging. I’ll explain.
I have multiple images of droplets (100+ droplets in each image, like those attached), those droplets are in different diameters and I simply need a way to automatically detect and measure diameter for all/most of the droplets in every image (which will later be used to produce a histogram).
This method will next be used on all my images, hence automatic/macros would be my ideal solution.
I’m using Fiji.
The problem I had when trying to solve it myself, is as you can see, many of the droplets are merged and/or in clusters and/or out of focus.
Since I can live with not measuring 100% of the droplets in each image - I have no problem if your suggestion will not be ideal and some of the droplets would not be measured…
Theoretically I can go and measure all diameters manually by hand, but I’m confident there is an automatic solution that can do it for me. Yet I was unable to find it on my own.
So this is why I need you guys.
Please let me know if you have some ideas that can help me and save me precious time.
Thank you for your time!