Hello there,

I’m new to the forum. I have been using ImageJ to analyse my data of Purkinje cell dendritic arbor. I was measuring the length of dendrites in binary and skeletonised image. I started using it yesterday after almost a year and figured out that the number of dendritic length in new measurements are completely different than earlier measurements. I couldn’t understand what went wrong and which parameters I put wrong or right there. I spent hours in figuring it out without any success.

Can someone help me here?

Here is how I did it?

Open the image =>make it binary=>skeletonise it=>measure

Thanks in advance!!