Measuring cilia

Hello,

Has anyone tried measuring cilia length in fluorescent images using CellProfiler? I would love if they would all lay down straight, but they can have all different kinds of curvature. Thanks for your help!

Tim

Hi Tim,

Without a more directed method for cilia in particular, offhand I could imagine this working:
(1) Segment the cilia (might be hard to segment these distinct from their source “ground”, but I can’t tell without images)
(2) Skeletonize (Morph module)
(2.5) Remove spurs (Morph)
(3) MeasureObjectSizeShape, to measure each cilia’s length, in pixels.

Hope that helps!
David