NND-CHN-P6.tif (324.4 KB)
I need to measure border to border distances (nearest neighbouring analysis) for my particles but, am not being successful. These are the steps and approaches that I have followed so far for the analysis:
- scaling, 8 bit adjustment, adjust threshold, analyse particles (show nothing, Display results checked), Plugins> Biovoxxel (2d particle distribution, average NND, etc.
- The problem with this option is that the centroid to centroid and border to border are giving very different results (e.g. 3 nm versus 20 nm) despite having small nanoparticles. Which makes me doubt my approach.
- Based on a comment in this topic (Measuring the distance between neighbor irregular particle ( lamellar)),
I tried scaling: 8 bit adjustment, adjust threshold, analyse particles (show count mask, everything unchecked), plugins> 3d> 3d distances> closest 2, borders
But, I do not get any reasonable answer.
I am new to this environment so, sorry if there is anything missing or wrong here.
I would highly appreciate help with this issue.