Measuring Axons and Ignoring Artifcats

cellprofiler

#1

Hello All,

I am trying to measure intact axons in cell profiler, but I need to ignore these artifacts that occurred while imaging with a Zeiss Celldiscoverer. The axons range from 300-2500 pixels in length, while the blobs are circular and range from 50-200 pixels in diameter. If anyone could shed some light on this, it would be greatly appreciated.


#2

Could you post an image?

I’m relatively new to CellProfiler so I may or may not be able to help, but I’ll give it a shot.