I have been extracting Area and Texture measurements and using these in Analyst to split my objects in categories. I then use these rules to classify my objects for all my experiments. Since I started using Cell Profiler 4.0.7 I can not use the Rules I have generated because all the Texture-derived measurements seem to not be recognised. I can not properly load my txt file with the rules on the pipeline as I get a message saying that “X measurement is not available at this stage of the pipeline. Consider editing the rules to match the available measurements or adding measurement modules to produce the measurements”. My pipelines however have not changed since I installed the latest CP version and Texture is still calculated in earlier stages of the pipeline.
Any ideas on why this may be happening?