I have a question about the values I got measuring the intensity of the Green channel in my primary (nuclei), secondari (cells) and tertiary objects (cytoplasm). Find, here attached, an Excel file with the values I am interested in: Integrated Intensity and Mean Intensity. As you can note, I got many outlyers values and the Integrated Intensity values are very high. Are these values normal?
I also attach the pipeline I used for the analysis and a sample image (Green and blue channels)
Note the photo corresponds to the data in the table that are highlighted in red.
I just would like to know if you find them consistent with the image and if the pipeline is well written.
Data_00.xlsx (50.3 KB)
Chiara_project.cpproj (552.5 KB)