I am quite new to ImageJ (and image analysis in general) and have been trying to do some measurements with the help of its documentation, but I am stuck at the segmentation stage. It would be great if you could give me some guidance or advice.
Here is an example of my pictures:
I am primarily interested in finding (1) the yolk-area and (2) the notochord length of the fish larvae. My goal is to be able to do this via Macro (possibly with minor manual modifications later).
So far I have tried the following for the yolk-sac area:
a) Subtract Background -> 8 bit -> Threshold (I fail to enclose the area of interest.)
b) Find Edge -> Binary -> (manual Erode -> Wand tracing tool) (I fail to select only the yolk-sac area as in the image it is not clearly enclosed.)
c) Manual Selection Brush Tool (It takes a lot of time to do so, but if the Batch process can initiate some process so that I only need to make minor adjustmnets to the selected region, it would also be very helpful.)
Any suggestions are highly appreciated. Thanks in advance!