Measurement of capillary diameter

Sample image and/or code

  • Upload an original image file here directly or share via a link to a file-sharing site (such as Dropbox) – (make sure however that you are allowed to share the image data publicly under the conditions of this forum).
  • Share a minimal working example of your macro code.

Background

  • What is the image about? Provide some background and/or a description of the image. Try to avoid field-specific “jargon”.

This is the pericyte cells (red) and vessels (green).

Analysis goals

  • What information are you interested in getting from this image?
    I want to get diameter of vessels.

Challenges

  • What stops you from proceeding?
    segmentation and diameter measurement.

  • What have you tried already?
    I tried segmentation of vessels using Image J and Icy.

  • Have you found any related forum topics? If so, cross-link them.
    No

  • What software packages and/or plugins have you tried?

I tried Image J and Icy.

Might want to look into some topics like these for FIJI.

Thank you very much. “Bounding rectangle option” is good for me. But I have multiple capillaries need to measure. But it seems just appear mean value of it. How can I get individual value of width and height?

Not sure, you should be getting one line per ROI.

I segmented below image using Weka. After that, how can I get each ROI like your picture? I am a beginner, sorry to ask this kind of question.

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I figure out. Thank you very much!

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@Henri_Pioneer
The information you are looking for will probably be there:

Good reading

Yes, it is! When I finished reading all the sentences! Thank you very much.