I am currently working with human cancer cell images from a three channel (RGB) acquisition performed on a 96-well plate. My pipeline includes:
CorrectIlluminationCalculate (x 3)
CorrectIlluminationApply (x 3)
IdentifySecondaryObjects (a new marker that I am trying to quantify)
Crop (x 4)
Tile (x 4)
Everything works fine, and I obtained processed images and csv files just fine. My question is what are the units of my intensity measurements (Mean, Median, Max, Min, StDev)? Are they integrals of the pixel intensities? Can someone please explain this to me?
And also, what is MADIntensity…?
Thank you in advance for replying, I appriciate it!