Measureing intensity

cellprofiler

#1

I want to measure the ratio of expression of protein, and I also want to get the inensity of it?
Both the picture of nuclear and the cell which proteins are expressed in it are all get!
How can I do?
thanks a lot!


#2

Hi Suidong,

Sorry I did not realize you had posted an English version of your question. Please look at the ExampleFlyImages on the Examples page to figure out how to measure the level of protein within a nucleus/cell. Then, read the help for the CalculateRatios module to figure out how to get your ratio.

Regards,
Mike


#3

thanks a lot for giving me the explaination!
I will try it again! If there r some questions I will ask you late !

tkank you a second time !!!

多谢了!!!