Measure total length of skeletonized image

Image Analysis
#1

Hi everyone!!! I am new CellProfiler user for image analysis. I want to analyze the picture in the shape of a branch. So I want to make a pipeline and get the total length and average length in the shape of a branch, but it doesn’t come from the pipeline I made.

Would you please make right this pipeline and calculate total length and average???
(In addition, I would like to thanks for coming out of the node junction.)Angiogenesis_sk.cpproj (432.3 KB)

#2

just a question, why are not using for this kind of analysis the analyze Skeleton tool of Fiji?
It seems to me it will do exactily what you are asking, and I used it several times for branches and skeleton analysis with good results.

#3

Thank you for reply @emartini