Sample image and/or code
This is a cross-section of rat muscle stained with anti-desmin antibody. This is a protein that is part of the whole cell’s structure, but forms clumps/aggregates in when its mutated.
I would like to find a way to measure how many desmin aggregates there are in a muscle cross section, how big their total area is and what their mean intensity is.
- There is a lot of “background”. I don’t know how to distinguish the aggregates (which I define by being thicker than the cell membrane and their signal of a higher intensity) and summarize their measurement.
- I have tried setting a threshold, but that only helps with signal intensity, not the size of the particles.
- I’m sorry if this is a very basic question, my ImageJ skills are very limited.
Any help is welcome! Thank you!