I have been trying to use this particular module for a couple days and I just can’t seem to make it work for me.
I’d like to measure bins starting from the edge of the nuclei (primary object) outwards into four or so bins. I have specified the secondary objects as endosomes and would like to measure their intensity within the bins. But I cannot seem to make this work. I have set the primary and secondary objects, I am using two different images (each has one object isolated).
Is there anything striking I could be doing wrong? Also, is there any way to visualize the outline of each bin?