Measure object intensity

Hello everyone,
I am trying to create a pipeline for the analysis of neurons.
I have 4 channels:
-Channel 1 is nuclei and I defined them as primary objects
-Channel 2 is subtype 1 of neurons and I defined them as secondary objects (secondary object 1)
-Channel 3 is subtype 2 of neurons and I defined them as secondary objects as well (secondary object 2)

Now I want to measure the intensity of channel 4 in both the secondary object 1 and 2 but it seems like the intensities are the same (one should give me 0), do you know what could be the problem or how I can specifically measure the intensity only in the subtypes and not in all cells?

Could you explain why you expect results in one object set to be 0? This seems unlikely unless the channel’s image is absolutely blank.

It might be helpful if you could upload a copy of the pipeline and a test image set so that we can take a look at this.