Measure intensity around a cell?

I am very new to analyzing images. I am trying to measure intensity around a cell but not inside the cell. I tried different ways but I keep getting stuck.
from an article by Christine Terryn et al.( Rapid Method of Quantification of Tight-Junction Organization Using Image Analysis.) I tried to follow how they did their image analysis but I got stuck.
I changed my image to grey scale 8bit
then did the grey level plot.
but the article says I determine threshold value from the plot but I can’t figure that out,
Also I tried using fractal analysis with the fraclac plugin on imagej but I don’t know how it works.

Dear @koko,

sorry that your post has been unanswered for so long!

It would be super helpful if you could provide a sample image and a more detailed description of what you are trying to do.


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